منابع مشابه
Latex immunoassay of urinary albumin.
A new non isotopic immunoassay based on latex particle agglutination has been developed for the determination of albumin in urine. The assay consists in incubating the urine sample for 30 min at 37 degrees C with latex particles coated with a specific antibody, followed by quantification of the resulting agglutination by particle counting or turbidimetry. The stability of antibody-coated partic...
متن کاملEnzyme immunoassay for urinary albumin.
We describe an enzyme-linked immunosorbent assay for urinary albumin, performed on microtiter plates with use of commercially available antisera and peroxidase conjugate. The assay range is 3-1000 micrograms/L, the sensitivity 625 pg. The method is suitable for measurement of albumin excretion in either normal or pathological urine. For 20 normal children, the range of urinary albumin excretion...
متن کاملOn the Composition of Urinary Albumin
A few findings which seem to be of importance may be pointed out:- Table I shows the analytical figures of serum-albumin, serum-globulin, and fibrin of the normal dog. The main difference between albumin and globulin appears in the relation of the precipitable to the non-precipitable total nitrogen and amino-nitrogen. Precipitable total nitrogen as well as amino-nitrogen is considerably larger ...
متن کاملCharacterization of immunochemically nonreactive urinary albumin.
BACKGROUND Conventional immunoassays underestimate the urinary albumin concentration because intact albumin in urine exists in two forms, immunoreactive and immunochemically nonreactive. METHODS Urinary albumin concentration measured by HPLC (which measures total albumin, i.e., the sum of immunoreactive albumin + immunochemically nonreactive albumin) or RIA was compared with densitometric ana...
متن کاملMicroplate measurement of urinary albumin and creatinine.
We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly simplifying calculations and improving sensitivity and accuracy. Measurement of c...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1927
ISSN: 0306-3283
DOI: 10.1042/bj0211109